Coding

Part:BBa_K1966001:Design

Designed by: Brandon Wilbanks   Group: iGEM16_UT-Knoxville   (2016-10-11)


lacPromoter-xylB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845


Design Notes

Constructed from synthetic DNA; inserted into pSB1C3 backbone via biobrick assembly.


Source

Pseudomonas putida TOL pathway (upper operon)

References